Virulence traits, agr typing, multidrug resistance patterns, and biofilm ability of MDR Staphylococcus aureus recovered from clinical and subclinical mastitis in dairy cows

• DOI: 10.1186/s12866-025-03870-3
• Publication Date: 2025-03-19T00:55:39Z
• Authors: Nada H. Eidaroos; Abdelazeem M. Alg...; Walaa Ismail Moha...; Asma Massad Alenzi; Saad Alghamdi; Ahmed Kabrah; Heba Sayed El-Mah...; Hamza M. Eid; Alaa Abd Algwad; Samar Ali Asfor; Heinrich Neubauer; Amira A. Moawad; Reham M. El-Tarabili
• Keywords: Subclinical infection

Bovine mastitis caused by Staphylococcus aureus is considered a public health threat globally. Herein, we aimed to investigate the occurrence, agr typing, antimicrobial resistance patterns, biofilm production, and PCR-based detection of virulence, biofilm, adhesion, enterotoxins genes S. strains recovered from clinical subclinical bovine mastitis. The prevalence in examined milk samples was 44.4%. Besides, 95% retrieved were identified as MRSA. all tested isolates producers. PCR revealed that 85% positive for I gene. Furthermore, clfB, clfA, fnbB, fnbA, cna detected with 100%, 80%, 60%, 55%, 30%, respectively. Also, coa gene (100%). 92.5% harbored lukF spa genes, In addition, hla, hlb, hlg hemolysin 70%, 50%, 35%, Among enterotoxin seb 30% strains. eno icaA Moreover, 15% MDR 8 agents mecA, ermC, ermB genes. As well, 12.5% carried ermB, tetK, tetM 5% XDR 11 existence MRSA hazard. coa, eno, icaA, lukF, spa, hla virulence are commonly associated predominant milk.