Identification and comprehensive analyses of the CBL and CIPK gene families in wheat (Triticum aestivum L.)

• DOI: 10.1186/s12870-015-0657-4
• Publication Date: 2015-11-04T14:19:03Z
• Authors: Tao Sun; Yan Wang; Meng Wang; Tingting Li; Yi Zhou; Xiatian Wang; Shuya Wei; Guangyuan He; Guangxiao Yang
• Keywords: Bimolecular fluorescence complementation; Common wheat

Calcineurin B-like (CBL) proteins belong to a unique group of calcium sensors in plant that decode the Ca2+ signature by interacting with CBL-interacting protein kinases (CIPKs). Although CBL-CIPK complexes have been shown play important roles responses various stresses plants, little is known about their functions wheat. A total seven TaCBL and 20 TaCIPK genes were amplified from bread wheat, Triticum aestivum cv. Chinese Spring. Reverse-transcriptase-polymerase chain reaction (RT-PCR) silico expression analyses showed expressed at different levels tissues, or maintained nearly constant during whole life cycle wheat plant. Some up- down-regulated expressions seed germination. Preferential interactions between TaCBLs TaCIPKs observed yeast two-hybrid bimolecular fluorescence complementation experiments. Analyses deletion series amino acid variations C-terminus provided new insights into specificity TaCBLs, indicated TaCBL–TaCIPK signaling pathway very complex because its hexaploid genome. The many responsive abiotic (salt, cold, simulated drought) abscisic treatment. Transgenic Arabidopsis plants overexpressing TaCIPK24 exhibited improved salt tolerance through increased Na+ efflux an enhanced reactive oxygen species scavenging capacity. These results contribute our understanding provide basis for selecting appropriate in-depth functional studies CBL–CIPK