The crucial role of PpMYB10.1 in anthocyanin accumulation in peach and relationships between its allelic type and skin color phenotype

• DOI: 10.1186/s12870-015-0664-5
• Publication Date: 2015-11-18T13:05:41Z
• Authors: Pham Anh Tuan; Songling Bai; Hideaki Yaegaki; Takayuki Tamura; Seisuke Hihara; Takaya Moriguchi; Kenji Oda
• Keywords: Skin Color

Red coloration of fruit skin is one the most important traits in peach (Prunus persica), and it mainly due to accumulation anthocyanins. Three MYB10 genes, PpMYB10.1, PpMYB10.2, PpMYB10.3, have been reported as regulators red anthocyanin biosynthesis fruit. In this study, contribution PpMYB10.1/2/3 was investigated Japanese cultivars, white-skinned 'Mochizuki' red-skinned 'Akatsuki'. We then relationships between allelic type PpMYB10.1 color phenotype 23 cultivars for future establishment DNA-marker. During development 'Akatsuki', observed only 'Akatsuki' late ripening stages concomitant with high mRNA levels last step gene leading accumulation, UDP-glucose:flavonoid-3-O-glucosyltransferase (UFGT). This also correlated expression level PpMYB10.1. Unlike PpMYB10.2/3 were low both throughout development. Moreover, revealed associated total leaves flowers Introduction into tobacco increased UFGT, resulting higher deeper transgenic flowers; however, overexpression did not alter content when compared wild-type flowers. Dual-luciferase assay showed that co-infiltration PpbHLH3 significantly activity PpUFGT promoter. found close two types, MYB10.1-1/MYB10.1-2, intensity coloration. a major regulator activates transcription. may be involved functions other than peach. The having MYB10.1-2 types resulted white color. By contrast, those MYB10.1-1 or MYB10.1-1/MYB10.1-2 respective pale These findings contribute clarifying molecular mechanisms generating gene-based markers linked phenotypes.