Contrasting transcriptional responses of PYR1/PYL/RCAR ABA receptors to ABA or dehydration stress between maize seedling leaves and roots

• DOI: 10.1186/s12870-016-0764-x
• Publication Date: 2016-04-21T09:44:46Z
• Authors: Wenqiang Fan; Mengyao Zhao; Suxin Li; Xue Bai; Jia Li; Haowei Meng; Zixin Mu
• Keywords: Osmotic shock

The different actions of abscisic acid (ABA) in the aboveground and belowground parts plants suggest existence a distinct perception mechanism between these organs. Although characterization soluble ABA receptors PYR1/PYL/RCAR as well core signaling components has greatly advanced our understanding perception, signal transduction, responses, environment-dependent organ-specific sensitivity to is less understood. By performing real-time quantitative PCR assays, we comprehensively compared transcriptional differences response or osmotic/dehydration stress maize (Zea mays L.) roots leaves. Our results demonstrated up-regulation transcript levels ZmPYLs homologous dimeric-type Arabidopsis by primary roots, whereas those monomeric-type were down-regulated. However, this trend was reversed leaves treated with via root medium. mRNA ZmPYL1-3 increased significantly subjected polyethylene glycol (PEG)-induced osmotic stress, ZmPYL4-11 transcripts either maintained at stable level only slightly. In detached dehydration, together ZmPYL5, ZmPYL6, ZmPYL10 ZmPYL11 decreased, ZmPYL4, ZmPYL7 ZmPYL8 increased. also showed that all evaluated PP2Cs SnRK2 quickly up-regulated stress; conversely they constant leaves, depending on isoforms within each family. There profile PYR/PYL/RCAR receptor gene expression suggesting are mainly involved transmission signals but primarily carry out function Given ZmPYL1 ZmPYL4 exhibit similar abundance under normal conditions, findings may represent novel for species-specific regulation expression. A difference preference presence exogenous versus stress-induced endogenous observed both roots. It appears perform their functions intensity-, duration-, species-, organ-, isoform-specific manner, leading plasticity adverse conditions and, thus, acclimation life land. These deepen diverse biological effects plant abiotic stimulus-perception level.