De novo sequencing and comparative transcriptome analysis of the male and hermaphroditic flowers provide insights into the regulation of flower formation in andromonoecious Taihangia rupestris

• DOI: 10.1186/s12870-017-0990-x
• Publication Date: 2017-02-28T01:47:13Z
• Authors: Weiguo Li; Lihui Zhang; Zhan Ding; Guodong Wang; Yandi Zhang; Hongmei Gong; Tianjun Chang; Yanwen Zhang
• Keywords: UniGene; KEGG; Illumina dye sequencing; Sequence assembly

Taihangia rupestris, an andromonoecious plant species, bears both male and hermaphroditic flowers within the same individual. However, establishment development of in remain poorly understood, due to limited genetic sequence information. To investigate potential molecular mechanism regulation flower formation, we used de novo RNA sequencing compare transcriptome profiles at early late developmental stages. Four cDNA libraries, including floral bud, flower, were constructed sequenced by using Illumina RNA-Seq method. Totally, 84,596,426 qualified reads obtained then assembled into 59,064 unigenes, which 24,753 unigenes annotated NCBI non-redundant protein database. In addition, 12,214, 7,153, 8,115 assigned 53 Gene Ontology (GO) functional groups, 25 Clusters Orthologous Group (COG) categories, 126 Kyoto Encyclopedia Genes Genomes (KEGG) pathways, respectively. By pairwise comparison unigene abundance between samples, identified 1,668 differential expressed genes (DEGs), 176 transcription factors (TFs) flowers. At stage, found 263 up-regulated 436 down-regulated buds, while 844 314 detected stage. GO KEGG enrichment analyses showed that a large number DEGs associated with wide range functions, cell cycle, epigenetic processes, development, biosynthesis unsaturated fatty acid pathway. Finally, real-time quantitative PCR was conducted validate present study. this study, data rare reported for first time. Comparative analysis revealed significant differences gene expression The would be helpful improve understanding underlying mechanisms formation unisexual plants.