Genome-wide analysis of the plant-specific PLATZ proteins in maize and identification of their general role in interaction with RNA polymerase III complex

• DOI: 10.1186/s12870-018-1443-x
• Publication Date: 2018-10-05T08:27:30Z
• Authors: Jiechen Wang; Chen Ji; Qi Li; Yong Zhou; Yongrui Wu
• Keywords: RNA polymerase II; Transcription

PLATZ proteins are a novel class of plant-specific zinc-dependent DNA-binding that classified as transcription factors (TFs). However, their common biochemical features and functions poorly understood. Here, we identified cloned 17 genes in the maize (Zea mays) genome. All ZmPLATZs were located nuclei, consistent with predicted role TFs. none was found to have intrinsic activation properties yeast. Our recent work shows FL3 (ZmPLATZ12) interacts RPC53 TFC1, two critical RNA polymerase III (RNAPIII) complex. Using yeast two-hybrid assay, determined seven other PLATZs interacted both whereas three had no protein-protein interaction these factors. The six either or TFC1. These findings indicate ZmPLATZ generally involved modulation RNAPIII-mediated small non-coding transcription. We also all members rice (Oryza sativa) Arabidopsis thaliana constructed Maximum likelihood phylogenetic tree for ZmPLATZs. resulting included 44 5 subfamilies. This study provides insight into understanding relationship, protein structure, expression pattern cellular localization maize. nine thirteen TFC1 current study, respectively. Overall, characterization functional analysis family will pave way regulation plant development.