FtMYB8 from Tartary buckwheat inhibits both anthocyanin/Proanthocyanidin accumulation and marginal Trichome initiation

• DOI: 10.1186/s12870-019-1876-x
• Publication Date: 2019-06-18T13:02:41Z
• Authors: Yunji Huang; Qi Wu; Shuang Wang; Jiaqi Shi; Qixin Dong; Panfeng Yao; Guannan Shi; Shuangxiu Xu; Renyu Deng; Chenglei Li; Hui Chen; Haixia Zhao
• Keywords: Anthocyanin; 0303 health sciences; Botany; Arabidopsis; FtMYB8; Trichomes; Trichome; Plants, Genetically Modified; Anthocyanins; 03 medical and health sciences; Gene Expression Regulation, Plant; QK1-989; Proanthocyanidin; Proanthocyanidins; Tartary buckwheat; Transcriptome; Sequence Alignment; Phylogeny; Research Article; Fagopyrum; Plant Proteins; Transcription Factors

Because flavonoids and trichomes play crucial roles in plant defence, their formation requires fine transcriptional control by multiple transcription factor families. However, little is known regarding the mechanism of the R2R3-MYB transcription factors that regulate both flavonoid metabolism and trichome development.Here, we identified a unique SG4-like-MYB TF from Tartary buckwheat, FtMYB8, which harbours the C2 repression motif and an additional TLLLFR repression motif. The expression profiles of FtMYB8 combined with the transcriptional activity of PFtMYB8 promoter showed that FtMYB8 mRNA mainly accumulated in roots during the true leaf stage and flowering stage and in bud trichomes and flowers, and the expression of this gene was markedly induced by MeJA, ABA and UV-B treatments but repressed by dark treatment. Overexpression of FtMYB8 in Arabidopsis reduces the accumulation of anthocyanin/proanthocyanidin by specifically inhibiting TT12 expression, which may depend on the interaction between FtMYB8 and TT8. Interestingly, this interaction may also negatively regulate the marginal trichome initiation in Arabidopsis leaves.Taken together, our results suggest that FtMYB8 may fine-tune the accumulation of anthocyanin/proanthocyanidin in the roots and flowers of Tartary buckwheat by balancing the inductive effects of transcriptional activators, and probably regulate trichome distribution in the buds of Tartary buckwheat.