Genome-wide identification and characterization of cucumber bHLH family genes and the functional characterization of CsbHLH041 in NaCl and ABA tolerance in Arabidopsis and cucumber

• DOI: 10.1186/s12870-020-02440-1
• Publication Date: 2020-06-11T17:02:57Z
• Authors: Jialin Li; Ting Wang; Jing Han; Zhonghai Ren
• Keywords: Cucumis; Synteny; Conserved sequence

Abstract Background The basic/helix-loop-helix (bHLH) transcription factor family exists in all three eukaryotic kingdoms as important participants biological growth and development. To date, the comprehensive genomic functional analyses of bHLH genes has not been reported cucumber ( Cucumis sativus L.). Results Here, a total 142 were identified classified into 32 subfamilies according to conserved motifs, phylogenetic analysis gene structures cucumber. sequences CsbHLH proteins highly based on results multiple sequence alignment analyses. chromosomal distribution, synteny analysis, duplications these CsbHLHs further analysed. Many elements related stress responsiveness plant hormones present promoter regions cis -element analysis. By comparing phylogeny Arabidopsis proteins, we found that clustered different clades proteins. expression selected under abiotic stresses (NaCl, ABA low-temperature treatments) five could simultaneously respond stresses. Tissue-specific profiles also In addition, 35S : CsbHLH041 enhanced tolerance salt transgenic seedlings, suggesting is an regulator response Lastly, interoperability network among was Conclusion This study provided good foundation for research functions regulatory mechanisms candidate resistance