Rice EARLY SENESCENCE 2, encoding an inositol polyphosphate kinase, is involved in leaf senescence

• DOI: 10.1186/s12870-020-02610-1
• Publication Date: 2020-08-26T04:12:03Z
• Authors: Shenglong Yang; Guonan Fang; Anpeng Zhang; Banpu Ruan; Hongzhen Jiang; Shilin Ding; Chaolei Liu; Yu Zhang; Noushin Jaha; Peng Hu; Zhengjin Xu; Zhenyu Gao; Jiayu Wang; Qian Qian
• Keywords: Senescence; Ethyl methanesulfonate

Abstract Background Early leaf senescence influences yield and quality by affecting plant growth development. A series of senescence-associated molecular mechanisms have been reported in rice. However, the complex genetic regulatory networks that control need to be elucidated. Results In this study, an early 2 ( es2 ) mutant was obtained from ethyl methanesulfonate mutagenesis (EMS)-induced mutational library for Japonica rice cultivar Wuyugeng 7 (WYG7). Leaves showed at seedling stage became severe tillering stage. The contents reactive oxygen species (ROS) significantly increased, while chlorophyll content, photosynthetic rate, catalase (CAT) activity decreased mutant. Moreover, genes which related senescence, ROS degradation were up-regulated, those associated with photosynthesis synthesis down-regulated compared WYG7. ES2 gene, encodes inositol polyphosphate kinase (OsIPK2), fine mapped a 116.73-kb region on chromosome 2. DNA sequencing revealed missense mutation, localized nucleus plasma membrane cells, expressed various tissues Complementation test overexpression experiment confirmed completely restored normal phenotype, rate increased comparable wild type. These results reveal new role OsIPK2 regulating therefore will provide additional evidence controlling senescence. Conclusions encoding is essential Further study facilitate dissection underlying growth.