Overexpression of the Salix matsudana SmAP2-17 gene improves Arabidopsis salinity tolerance by enhancing the expression of SOS3 and ABI5

• DOI: 10.1186/s12870-022-03487-y
• Publication Date: 2022-03-07T06:03:42Z
• Authors: Yanhong Chen; Yuanhao Dai; Yixin Li; Jie Yang; Yuna Jiang; Guoyuan Liu; Chunmei Yu; Fei Zhong; Bolin Lian; Jian Zhang
• Keywords: 0301 basic medicine; Overexpression; AP2/ERF; Research; Botany; Arabidopsis; Salix; Salt Tolerance; 15. Life on land; Genes, Plant; Plants, Genetically Modified; Salt Stress; Salix matsudana; Plant Breeding; 03 medical and health sciences; Gene Expression Regulation, Plant; QK1-989; Salt tolerance; Sequence Analysis; Phylogeny; Transcription Factors

Abstract Background Salix matsudana (Koidz.) is a widely planted ornamental allotetraploid tree species. Genetic engineering can be used to enhance the tolerance of this species soil salinization, endowing varieties with ability grow along coastlines, thereby mitigating afforestation and protecting environment. The AP2/ERF family transcription factors (TFs) plays multidimensional roles in plant biotic/abiotic stress development. In study, we cloned SmAP2-17 gene performed functional analysis its role salt tolerance. This study aims identify key genes for future breeding stress-resistant . Results was predicted homolog AP2-like ethylene-responsive factor ANT isoform X2 from Arabidopsis , ORF 2058 bp encoding an estimated protein 685 amino acids containing two conserved AP2 domains (PF00847.20). had constitutive expression pattern localized nucleus. overexpression native CDS sequence did not increase because reduced level ectopic potentially caused by salt-induced RNAi. Transgenic lines high optimized under showed enhanced salt. Moreover, general marker important signaling genes, including RD29A ABI5 SOS3, AtHKT1, RBohF, were upregulated -overexpressed lines, levels consistent that or Promoter activity using dual luciferase could bind promoters SOS3 activate their expression, which regulating Conclusions isolated positive regulator improves resistance transgenic plants upregulating genes. provides potential resource generation salt-resistant genetic engineering.