Suppression of GhGLU19 encoding β-1,3-glucanase promotes seed germination in cotton

• DOI: 10.1186/s12870-022-03748-w
• Publication Date: 2022-07-22T12:04:30Z
• Authors: Haitang Wang; Xuesong Zhou; Chuchu Liu; Weixi Li; Wangzhen Guo
• Keywords: Callose; Imbibition; Gossypium; Integument

In eudicots, germination begins with water uptake by the quiescent dry seed and is greatly related to permeability of micropyle enriched callose layers. Once imbibition starts, seeds undergo a cascade physiological, biochemical, molecular events initiate cellular activities. However, effects on following metabolic during are largely unknown. Cotton (Gossypium hirsutum) eudicot plant natural fiber edible oil production for humans. Here, we addressed this question examining role GhGLU19, gene encoding β-1,3-glucanase, in cotton germination.GhGLU19 belongs subfamily B was expressed predominately imbibed early seedlings. Compared wild type, GhGLU19-suppressing GhGLU19-overexpressing transgenic lines showed higher lower percentage, respectively. Callose more at inner integument (ii) than that embryo coat seeds. lines, ii increased brought about prolonged process imbibition. Both proteomic transcriptomic analysis revealed contrary glycolysis pyruvate metabolism decreased, abscisic acid (ABA) biosynthesis genes were downregulated lines. Also, endogenous ABA significantly decreased line while line.Our results demonstrate suppression GhGLU19 improves via accumulating integument, modulating metabolism, decreasing biosynthesis. This study provides potential way improving percentage production, other crops.