Physcomitrium patens CAD1 has distinct roles in growth and resistance to biotic stress

• DOI: 10.1186/s12870-022-03892-3
• Publication Date: 2022-11-08T03:02:26Z
• Authors: Shan Jiang; Xu Tian; Xiaolong Huang; Jiankang Xin; Huiqing Yan
• Keywords: Cinnamyl-alcohol dehydrogenase; Biotic stress

Physcomitrium patens provides an evolutionary link between green algae and vascular plants. Although the genome of P. includes orthologs all core lignin biosynthetic enzymes, occurrence in moss is very controversial. Besides, little information available about enzymes to date. For example, cinnamyl alcohol dehydrogenase (CAD) a crucial enzyme that catalyzes last step pathway, suggesting ideal way study process. By investigating functions CAD evolution, this will elucidate roles lignin-like early stage land colonization.CAD multigene family composed four genes. The PpCADs contain conserved glycine-rich domain catalyze NADPH-dependent reduction their corresponding alcohols, indicating have potential synthesize monolignols by bioinformatics analysis. Even though PpCAD1 could produce theory, no conventional monomer was detected cell wall or cytoplasm PpCAD1_OE However, phenylpropanoids were promoted transformants modify gametophore architecture development, making distribution phyllids more scarcity colony giant, possibly due enhanced expression AUX-IAA family. transcripts at least one gene encoding pathway increased In addition, inhibited Botrytis cinerea assault mainly instead influencing defense genes pathogenesis-related 10 (PR10) nonexpresser PR 1 (NPR1). Likewise, ectopic Arabidopsis led significant increase content, exhibiting chunky roots, robust seedlings, advanced flowering, efficient resistance against pathogens.PpCAD occurs than copy, functional divergence ancestral plant. monolignol biosynthesis has homologous with Despite monolignol, gametophore, intermediate metabolites had during evolution terrestrial results inferred non-vascular plant played stem elongation pathogens conquest land.