Transcriptome profiling provides insights into leaf color changes in two Acer palmatum genotypes

• DOI: 10.1186/s12870-022-03979-x
• Publication Date: 2022-12-16T07:02:48Z
• Authors: Lu Zhu; Jing Wen; Qiuyue Ma; Kunyuan Yan; Yiming Du; Zhu Chen; Xiaoyu Lu; Jie Ren; Yuelan Wang; Shushun Li; Qianzhong Li
• Keywords: Profiling (computer programming)

Ornamental trees with seasonally-dependent leaf color, such as Acer palmatum, have gained worldwide popularity. Leaf color is a main determinant of the ornamental and economic value A. palmatum. However, molecular mechanisms responsible for changes remain unclear.We chose palmatum cultivars yellow ('Jinling Huangfeng') red Danfeng') leaves ideal material studying complex metabolic networks variations in coloration. The 24 libraries obtained from four different time points growth 'Jinling Huangfeng' Danfeng' was subjected to Illumina high-throughput sequencing. We observed that difference cyanidin delphinidin content primary reason behind varying coloration leaves. Transcriptomic analyses revealed 225,684 unigenes, Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analysis differentially expressed genes (DEGs) confirmed they were involved 'anthocyanin biosynthesis.' Eighteen structural anthocyanin biosynthesis thought be related accumulation, whereas 46 MYBs, 33 basic helix-loop-helixs (bHLHs), 29 WD40s presumed regulating biosynthesis. Based on weighted gene co-expression network (WGCNA), three candidate (ApRHOMBOID, ApMAPK, ApUNE10) screened significant association module correlation coefficient (r2) 0.86.In this study, two genotypes analyzed. These findings provide novel insights into suggest pathways targeted genetic improvements