Genome-wide analysis of CHYR gene family and BnA03.CHYR.1 functional verification under salt stress in Brassica napus L.

• DOI: 10.1186/s12870-025-06343-x
• Publication Date: 2025-03-20T07:47:54Z
• Authors: Yanli Guo; Qingxiao Ren; Manman Song; Xiangxiang Zhang; Heping Wan; Fei Liu
• Keywords: Brassicaceae; Silique

Abstract Brassica napus , an allotetraploid used as oilseed crop, vegetable, or feed possesses significant economic and medicinal value. Although the CHYR gene family has been functionally characterised in various aspects of plant growth, development, stress responses, its systematic investigation B. is lacking. In contrast to seven genes ( AtCHYR1-AtCHYR7 ) identified Arabidopsis thaliana nine orthologues were detected rapa oleracea while 24 found . This discrepancy consistent with established triplication events that occurred during Brassicaceae evolution. Phylogenetic analysis indicated CHYRs could be categorised into three distinct groups. Among these, BnCHYRs contained conserved domains, including CHY-zinc finger, C3H2C3-type RING finger zinc ribbon domains. Group III members featured additional one hemerythrin domains their N-terminal regions. Each BnCHYR group shared similar patterns distribution Our results revealed selected eight up-regulated following heat treatment, exhibiting varying expression response salt, cold, drought seedling stage. Expression several significantly induced by more abiotic stressors. BnA03.CHYR.1 was salt repressed polyethylene glycol treatment. localised nucleus cytoplasm, overexpression A. enhanced tolerance stress. provide a comprehensive elucidating biological role adaptive responses plants