Fibroblast activation protein-α-expressing fibroblasts promote the progression of pancreatic ductal adenocarcinoma

• DOI: 10.1186/s12876-015-0340-0
• Publication Date: 2015-09-01T09:47:55Z
• Authors: Tomoya Kawase; Yumiko Yasui; Sohji Nishina; Yuichi Hara; Izumi Yanatori; Yasuyuki Tomiyama; Yoshihiro Nakashima; Koji Yoshida; Fumio Kishi; Masafumi Nakamura; Keisuke Hino
• Keywords: Pancreatic carcinoma

Pancreatic ductal adenocarcinoma (PDAC) is characterized by an extensive desmoplastic stromal response. Fibroblast activation protein-α (FAP) best known for its presence in cancer-associated fibroblasts (CAFs). Our aim was to assess whether FAP expression associated with the prognosis of patients PDAC and investigate how expressing CAFs contribute progression PDAC. immunohistochemically assessed 48 specimens. We also generated a fibroblastic cell line stably FAP, examined effect FAP-expressing on invasiveness cycle MiaPaCa-2 cells (a pancreatic cancer line). Stromal detected 98 % (47/48) specimens PDAC, intensity being weak 16, moderate 19, strong 12 specimens, but not 3 control noncancerous Patients or had significantly lower cumulative survival rates than those negative (mean time; 352 vs. 497 days, P = 0.006). Multivariate analysis identified as one factors The positively correlated histological differentiation (P < 0.05). promoted more intensively FAP. Coculture activated shift compared coculture Furthermore, inactivated retinoblastoma (Rb) protein, inhibitor progression, promoting phosphorylation Rb. present vitro results association clinical outcomes provide us better understanding