Impact of nutrition and rotavirus infection on the infant gut microbiota in a humanized pig model

• DOI: 10.1186/s12876-018-0810-2
• Publication Date: 2018-06-22T01:29:12Z
• Authors: Anand Kumar; Anastasia N. Vlasova; Loic Deblais; Huang-Chi Huang; Asela Wijeratne; Sukumar Kandasamy; David D. Fischer; Stephanie N. Langel; Francine Chimelo ...; Moyasar A. Alhamo; Lulu Shao; Linda J. Saif; Gireesh Rajashekara
• Keywords: Viral Shedding

Human rotavirus (HRV) is a major cause of viral gastroenteritis in infants; particularly developing countries where malnutrition prevalent. Malnutrition perturbs the infant gut microbiota leading to sub-optimal functioning immune system and further predisposing infants enteric infections. Therefore, we hypothesized that exacerbates disease severity infants.In present study, used neonatal germ free (GF) piglets transplanted with two-month-old human infant's fecal (HIFM) on protein deficient sufficient diets. We report effects malnourishment HRV infection HIFM pig feces, intestinal systemic tissues, using MiSeq 16S gene sequencing (V4-V5 region).Microbiota analysis indicated transplantation resulted microbial composition pigs similar original feces. This model was then understand interconnections between diversity, diet, infection. Post infection, diet had lower body weights, developed more severe diarrhea increased virus shedding compared diet. However, induced pronounced non-colonized GF either or suggesting alone moderated infected showed diversity tissues; whereas, greater observed tissues fed diet.These results suggest proper nourishment improves quality intestines, alleviates probability translocation potential opportunistic pathogens/pathobionts. In conclusion, our findings support role for nutrition limiting diseases.