RAPD-PCR reveals genetic polymorphism among Leishmania major strains from Tunisian patients

• DOI: 10.1186/s12879-015-1010-0
• Publication Date: 2015-07-13T15:52:58Z
• Authors: Rihab Yazidi; Jihene Bettaieb; Wissem Ghawar; Kaouther Jaouadi; Sana Châabane; Amor Zaatour; Afif Ben Salah
• Keywords: Genetic Variability; Parasitology; Dendrogram

Zoonotic cutaneous leishmaniasis caused by Leishmania (L.) major is endemoepidemic in the Center and South of Tunisia. The clinical course disease varies widely among different patients geographic regions. Although genetic diversity L. parasites has been suggested as a potential factor influencing their pathogenic variability, little information on polymorphism strains available literature. This work aimed to estimate variability within isolates major.Our sample comprised 39 (confirmed restriction fragment length typing) from experiencing same manifestations but living regions Tunisia where endemic. Random amplified polymorphic DNA (RAPD) PCR marker was estimated calculating Nei Li's distances an analysis molecular variance (AMOVA).Analysis revealed high level (43 %) them. AMOVA indicated that highest (99 existed study regions.Our results heterogeneous profile for with similar occurring geographical Additional patients, insect vectors, reservoir hosts endemic foci should be collected further analysis.