High heterogeneity of fecal carriage extended-spectrum beta-lactamase-producing E. coli isolated from iranian community and clinical settings

• DOI: 10.1186/s12879-022-07304-7
• Publication Date: 2022-03-31T13:21:48Z
• Authors: Shadi Aghamohammad; Vajihe Sadat Nikbin; Farzad Badmasti; Fereshteh Shahche...
• Keywords: MacConkey agar; Carriage; Beta-lactamase; Cefepime; Aztreonam; Medical microbiology

Abstract Background Extended-spectrum beta-lactamase-producing enterobacteria (ESBL-PE) in carriers have become a global health problem. Using molecular typing techniques, including PFGE, could be useful to determine the source of bacterial dissemination. The current study aimed investigate intestinal carriage ESBL-producing E. coli (ESBL-EC) and clonal relatedness among ESBL-EC isolated from hospitalized outpatient fecal Iran. Methods A total 120 rectal swabs were collected; 50.8% (61/120) intensive care unit (ICU) inpatients 49.2% (59/120) outpatients. MacConkey agar enriched with cefotaxime was used screen ESBL-EC. PCR assays performed detect ESBL carbapenemase genes. Pulse-fields gel electrophoresis (PFGE) assess relatedness. Results Totally, 60.0% (72/120) carrier for rates resistance against ceftazidime cefepime 90.2% (65/72) 93.0% (67/72), respectively. bla CTX-M-15 , TEM SHV NDM-1 OXA-48 IMP (65/72), 50.0% (36/72), 5.5% (4/72), 4.1% (3/72), (3/72) 1.3% (1/72), Based on cut-off 80%, 69 isolates categorized 10 mini-cluster 47 considered as singletons. Discussion High heterogeneity suggests that this bacterium probably has different Screening hospitals communities help infection control program public health.