Aberrant expression of GSTM5 in lung adenocarcinoma is associated with DNA hypermethylation and poor prognosis

• DOI: 10.1186/s12885-022-09711-0
• Publication Date: 2022-06-21T20:02:40Z
• Authors: Xuewei Hao; Jun Zhang; Guoyou Chen; Weiwei Cao; Hongyang Chen; Shuo Chen
• Keywords: Surgical oncology

Glutathione-S transferases (GSTs) comprise a series of critical enzymes involved in detoxification endogenous or xenobiotic compounds. Among several GSTs, Glutathione S-transferases mu (GSTM) has been implicated number cancer types. However, the prognostic value and potential functions GSTM family genes have not investigated lung adenocarcinoma (LUAD).We examined expression GSTM5 LUAD identified associations among expression, clinicopathological features, survival data from Cancer Genome Atlas (TCGA). The correlation between DNA methylation its was analyzed using MEXPRESS tool UCSC Xena browser. status promoter region cells measured by methylation-specific PCR (MSP). After 5-aza-2'-deoxycytidine treatment cells, GSTM5, cell proliferation migration were assessed RT-PCR, CCK-8 transwell assays, respectively.The results showed that abnormally down-regulated patients' tissues, patients with low level had significantly shorter OS. Cox regression analyses revealed associated overall (OS) patients, which an independent indicator terms OS (hazard ratio: 0.848; 95% CI: 0.762-0.945; P = 0.003). In addition, we found hypermethylated tumor tissue compared adjacent normal average moderately correlated expression. Moreover, also gene 5-Aza-CdR can restore inhibit migration. Finally, Gene Set Enrichment Analysis (GSEA) related to repair pathways.Our demonstrate high may act as novel putative molecular target for LUAD.