Regulation of TERRA on telomeric and mitochondrial functions in IPF pathogenesis

• DOI: 10.1186/s12890-017-0516-1
• Publication Date: 2017-12-02T03:51:03Z
• Authors: Yulin Gao; Jinjin Zhang; Yuxia Liu; Songzi Zhang; Youlei Wang; Bo Liu; Huizhu Liu; Rongrong Li; Changjun Lv; Xiaodong Song
• Keywords: Male; 0301 basic medicine; Aging; Apoptosis; Diseases of the respiratory system; Mice; 03 medical and health sciences; lncRNA; Animals; Humans; Aged; Cell Proliferation; RC705-779; Superoxide Dismutase; TERRA; Hydrogen Peroxide; Telomere; Middle Aged; Catalase; Idiopathic Pulmonary Fibrosis; Mitochondria; 3. Good health; IPF; A549 Cells; Case-Control Studies; Female; RNA Interference; RNA, Long Noncoding; Reactive Oxygen Species; Research Article

Aging is a known risk factor of idiopathic pulmonary fibrosis (IPF). However, the pathogenic mechanisms underlying effects advanced aging remain largely unknown. Telomeric repeat-containing RNA (TERRA) represents type long noncoding RNA. In this study, regulatory roles TERRA on human telomeres and mitochondria IPF epithelial injury model were identified. Blood samples collected from patients with (n = 24) matched control individuals 24). The significance clinical research expression correlated was assessed. levels in vivo vitro determined through quantitative real-time polymerase chain reaction analysis. Telomerase activity observed using fluorescent TRAP assay kit. functions telomeres, mitochondria, associated genes analyzed interference TERRA. significantly increased peripheral blood mononuclear cells patients. also exhibited direct inverse correlation percentage predicted force vital capacity, which physiological indicator fibrogenesis during progression. This finding confirmed vitro. can ameliorate telomeres; mitochondria; genes; components such as telomerase reverse transcriptase, telomerase, cell nuclear antigen, cyclin D1; mitochondria-associated E genes, including MMP Bcl-2 family. improve oxidative-stress-associated reactive oxygen species, superoxide dismutase, catalase, apoptosis-related cytochrome c, caspase-9, caspase-3. regulation pathogenesis identified for first time. results may provide valuable insights discovery novel biomarker or therapeutic approach treatment.