High CENPA expression in papillary renal cell carcinoma tissues is associated with poor prognosis

• DOI: 10.1186/s12894-022-01106-4
• Publication Date: 2022-09-26T09:08:02Z
• Authors: Junwu Li; Qinke Li; Yang Yuan; Yiteng Xie; Yuanfeng Zhang; Ronggui Zhang
• Keywords: Nomogram

This work focused on investigating the relation of centromeric protein A (CENPA) gene expression with prognosis papillary renal cell carcinoma (PRCC).We obtained data from PRCC cases in TCGA. Thereafter, CENPA levels between paired and matched non-carcinoma samples were analyzed by Wilcoxon rank-sum test, while relations clinicopathological characteristics level examined logistic regression test. The prognostic value was assessed plotting receiver operating feature curve (ROC) calculating area under (AUC). In addition, survival through Kaplan-Meier (KM) Cox analyses. After dividing total number patients into trial cohort validation a ratio 7:3, we constructed nomogram according to multivariate results for predicting how affected patient used calibration verify its accuracy both cohorts. We also determined within cancer immunohistochemistry (IHC). Finally, utilized functional enrichment identifying key pathways related differentially expressed genes (DEGs) up-regulation down-regulation.CENPA enhanced tissues compared healthy counterparts (P < 0.001). pathological TNM stage clinical 0.05). Meanwhile, ROC curves indicated that had remarkable diagnostic capacity PRCC, can significantly improve predictive PRCC. As revealed KM curves, associated poor those down-regulation (Risk ratio, RR = 3.07, 95% CI: 1.58-5.97, P meantime, univariate as well analysis showed an independent association overall (OS, 0.05) demonstrated superior ability IHC increased positive rate controls. annotations, enriched neuroactive ligand receptor interactions, cytokine extracellular matrix regulators, glycoproteins nuclear matrisome.CENPA increases samples, which predicts dismal survival. may become molecular marker therapeutic target patients.