Development of phytase-expressing chlamydomonas reinhardtii for monogastric animal nutrition

• DOI: 10.1186/s12896-016-0258-9
• Publication Date: 2016-03-12T01:48:24Z
• Authors: Fernanda Erpel; Franko Restovic; Patricio Arce-Joh...
• Keywords: Phytase; Monogastric; Chlamydomonas reinhardtii; Animal Feed; Aspergillus niger

In plant-derived animal feedstuffs, nearly 80 % of the total phosphorus content is stored as phytate. However, phytate poorly digested by monogastric animals such poultry, swine and fish, they lack hydrolytic enzyme phytase; hence it regarded a nutritionally inactive compound from phosphate bioavailability point view. addition, also chelates important dietary minerals essential amino acids. Therefore, supplementation with bioavailable exogenous phytases are required to achieve optimal growth. order simplify obtaining application processes, we developed phytase expressing cell-wall deficient Chlamydomonas reinhardtii strain.In this work, transgenic microalgae fungal be used food supplement for animals. A codon optimized Aspergillus niger PhyA E228K (mE228K) improved performance at pH 3.5 was transformed into plastid genome in expression. We engineered plastid-specific construction harboring mE228K gene, which allowed us obtain high expression level lines measurable vitro activity. Both wild-type strains were selected, latter suitable model digestion. The enzymatic activity approximately 5 units per gram dry biomass 37 °C, similar physiological conditions economically competitive use commercial activities.A reference basis future biotechnological provided work. gastrointestinal temperature pellet formation developed. Moreover, associated costs strain would between US$5 US$60 ton feedstuff, US$2 feedstuffs commercially available phytases. Our data provide evidence phytate-hydrolyzing additive without need protein purification.