Methanandamide diminish the Porphyromonas gingivalis lipopolysaccharide induced response in human periodontal ligament cells

• DOI: 10.1186/s12903-020-01087-6
• Publication Date: 2020-04-15T16:03:52Z
• Authors: Fengqiu Zhang; Burcu Özdemir; Phuong Quynh Nguyen; Oleh Andrukhov; Xiaohui Rausch-Fan
• Keywords: Anandamide; Periodontal fiber

The endocannabinoid system is involved in the regulation of periodontal tissue homeostasis. Synthetic cannabinoid methanandamide (Meth-AEA) has improved stability and affinity to receptors compared its endogenous analog anandamide. In present study, we investigated effect on production pro-inflammatory mediators primary human ligament cells (hPdLCs).hPdLCs were treated with Meth-AEA for 24 h, resulting interleukin (IL)-6, IL-8, monocyte chemotactic protein (MCP)-1 was measured absence or presence Porphyromonas gingivalis lipopolysaccharide (LPS). Additionally, proliferation/viability hPdLCs by MTT method.Methanandamide at a concentration 10 μM significantly inhibited P. LPS induced IL-6, MCP-1. Basal IL-6 IL-8 slightly enhanced Meth-AEA. No basal MCP-1 observed. concentrations up did not affect hPdLCs, but it 30 μM.Our study suggests that inflammatory response could be influenced activation system, which might potentially progression disease.