Proangiogenesis effects of compound danshen dripping pills in zebrafish

• DOI: 10.1186/s12906-022-03589-y
• Publication Date: 2022-04-22T15:02:54Z
• Authors: Yang-Xi Hu; Hong-Min You; Chang-Zhen Ren; Bo-Wen Hu; Lu-Jun Zhang; Yan-Da Zhang; Zhi-Qing He; Ru Ding; Zhi-Fu Guo; Chun Liang
• Keywords: Panax notoginseng

Abstract Background The compound Danshen Dripping Pill (CDDP), which is a mixture of extracts from Radix Salviae and Panax notoginseng , patented traditional Chinese medicine that widely used in multiple countries for relieving coronary heart disease (CHD), but its pharmacological mechanism has not been fully elucidated. In this study, we screened the key pathways targets CDDP act on CHD using network pharmacology-based strategy, angiogenic activity was directly visually investigated zebrafish embryos vivo. Methods potential therapeutic were predicted through bioinformatics analysis. proangiogenic effects examined vascular sprouting assays subintestinal vessels (SIVs) optic arteries (OAs) as well injury intersegmental (ISVs). Pharmacological experiments applied to confirm pathway involved. Results Sixty-five identified enriched PI3K/AKT VEGF/VEGFR . An vivo study revealed promoted angiogenesis SIVs OAs dose-dependent manner relieved impairments ISVs induced by lenvatinib, VEGF receptor kinase inhibitor (VRI). addition, Vegfaa Kdrl expression significantly upregulated after treatment. Furthermore, effect could be abolished inhibitors. Conclusions effect, involves signaling pathways. These results suggest new insight into cardiovascular protective CDDP.