Anti-tumor and antioxidant activity of kaempferol-3-O-alpha-L-rhamnoside (Afzelin) isolated from Pithecellobium dulce leaves

• DOI: 10.1186/s12906-022-03633-x
• Publication Date: 2022-06-22T13:03:10Z
• Authors: Masuma Akter; Mst. Shahnaj Parvin; Md. Mahadi Hasan; Md. Aziz Abdur Ra...; Md. Ekramul Islam
• Keywords: Ehrlich ascites carcinoma

Pithecellobium dulce (Roxb.), an evergreen medium-sized, spiny tree which have vast nutritional values and widely used in ayurvedic medicines home remedies. The plant has also been a rich source of biologically active compounds. present study was designed to isolate pure compound from ethyl acetate fraction methanol extract leaves know the efficacy as antioxidant well its anti-tumor activity on Ehrlich ascites carcinoma cell (EAC). METHODS: were extracted with fractionated different solvents. isolation carried out by column chromatography (EAF) structure revealed 1H-NMR 13C NMR. investigated scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals inhibition oxidative damage pUC19 plasmid DNA, hemolysis lipid peroxidation induced water-soluble radical initiator 2,2'-azo (2-asmidinopropane) dihydrochloride (AAPH) human erythrocytes. In vivo evaluated determining viable tumor count, hematological profiles experimental mice along observing morphological changes EAC cells fluorescence microscope.The isolated kaempferol-3-O-alpha-L-rhamnoside effectively inhibited AAPH oxidation DNA erythrocyte model per stronger DPPH activity. assay, at dose 50 mg/kg body weight exhibit about 70.89 ± 6.62% growth inhibition, whereas standard anticancer drug vincristine showed 77.84 6.69% inhibition.The may great importance therapeutic agent preventing biomolecules use chemotherapy.