Transcriptome, proteome and draft genome of Euglena gracilis

• DOI: 10.1186/s12915-019-0626-8
• Publication Date: 2019-02-07T11:04:17Z
• Authors: ThankGod E. Ebenezer; Martin Zoltner; Alana Burrell; Anna Nenarokova; Anna M. G. Novák ...; Binod Prasad; Petr Soukal; Carlos Santana-Mo...; Ellis O’Neill; Nerissa N. Nankis...; Nithya Vadakedath; Viktor Daiker; Samson Obado; Sara Silva-Pereira; Andrew P. Jackson; Damien P. Devos; Julius Lukeš; Michael Lebert; Sue Vaughan; Vladimίr Hampl; Mark Carrington; Michael L. Ginger; Joel B. Dacks; Steven Kelly; Mark C. Field
• Keywords: Euglena gracilis; Proteome

Photosynthetic euglenids are major contributors to fresh water ecosystems. Euglena gracilis in particular has noted metabolic flexibility, reflected by an ability thrive a range of harsh environments. E. been popular model organism and considerable biotechnological interest, but the absence gene catalogue hampered both basic research translational efforts. We report detailed transcriptome partial genome for Z1. The nuclear is estimated be around 500 Mb size, encodes over 36,000 proteins possesses less than 1% coding sequence. Annotation sequences indicates highly sophisticated endomembrane system, RNA processing mechanisms contributions from several photosynthetic lineages. Multiple families, including likely signal transduction components, have massively expanded. Alterations protein abundance controlled post-transcriptionally between light dark conditions, surprisingly similar trypanosomatids. Our data provide evidence that eukaryotes contributed genome, support 'shopping bag' hypothesis plastid acquisition. also suggest possess unique regulatory achieving extreme adaptability, through paralog expansion