Effects of corticosterone on the metabolic activity of cultured chicken chondrocytes

• DOI: 10.1186/s12917-015-0398-5
• Publication Date: 2015-04-07T14:54:19Z
• Authors: Hua Zhang; Zhenlei Zhou; Jingwen Luo; Jiafa Hou
• Keywords: Corticosterone; Metabolic activity

Corticosterone is one of the most crucial glucocorticoids (GCs) in poultry. Our previous study shows that corticosterone can retard longitudinal growth bones by depressing proliferation and differentiation chondrocytes broilers. The present was designed to investigate whether affect development synthesis collagen vitro. were isolated from proximal tibial plates 6-week-old broiler chickens cultured with different doses for 48 h. Then cell viability, alkaline phosphatase (ALP) activity expression parathyroid hormone-related peptide (PTHrP) type X (Col X) detected. At 10−9-10−6 M concentration, significantly inhibited viability chondrocytes, as indicated decreases ALP expression. Conversely, there completely opposite effect at 10−10 M. In addition, PTHrP downregulated 10−6 10−8 M, upregulated results suggested regulated chicken performance depending on its concentration high concentrations inhibiting light promoting them, these roles may be part mediated through PTHrP.