Randomized comparison of next-generation sequencing and array comparative genomic hybridization for preimplantation genetic screening: a pilot study

• DOI: 10.1186/s12920-015-0110-4
• Publication Date: 2015-06-22T14:34:33Z
• Authors: Zhihong Yang; James Lin; John Zhang; Wai Ieng Fong; Pei Li; Rong Zhao; Xiaohong Liu; William Podevin; Yanping Kuang; Jiaen Liu
• Keywords: Comparative genomic hybridization; Advanced maternal age; Human genetics

Recent advances in next-generation sequencing (NGS) have provided new methods for preimplantation genetic screening (PGS) of human embryos from vitro fertilization (IVF) cycles. However, there is still limited information about clinical applications NGS IVF and PGS (IVF-PGS) treatments. The present study aimed to investigate the effects on pregnancy implantation outcomes patients comparison array comparative genomic hybridization (aCGH) screening.This was performed two phases. Phase I evaluated accuracy aneuploidy aCGH. Whole-genome amplification (WGA) products (n = 164) derived previous IVF-PGS cycles 38) were retrospectively analyzed with NGS. results then compared those II further between aCGH patients. A total 172 at mean age 35.2 ± 3.5 years randomized into groups: 1) (Group A): 86) had screened 2) B): For both groups, blastocysts vitrified after trophectoderm biopsy. One euploid thawed transferred individual primarily based results. Ongoing rates groups.NGS detected all types aneuploidies accurately a 100 % 24-chromosome diagnosis consistency highly validated method. Moreover, identified transfer resulted similarly high ongoing (74.7 vs. 69.2 %, respectively, p >0.05). observed also comparable groups (70.5 66.2 >0.05).While has been recently introduced assist patients, this first efficiency With resulting rates, demonstrated an efficient, robust high-throughput technology PGS.