Signature RNAS and related regulatory roles in type 1 diabetes mellitus based on competing endogenous RNA regulatory network analysis

• DOI: 10.1186/s12920-021-00931-0
• Publication Date: 2021-05-18T09:02:48Z
• Authors: Qinghong Shi; Hanxin Yao
• Keywords: Competing Endogenous RNA; Gene regulatory network

Abstract Background Our study aimed to investigate signature RNAs and their potential roles in type 1 diabetes mellitus (T1DM) using a competing endogenous RNA regulatory network analysis. Methods Expression profiles of GSE55100, deposited from peripheral blood mononuclear cells 12 T1DM patients 10 normal controls, were downloaded the Gene Omnibus uncover differentially expressed long non-coding (lncRNAs), mRNAs, microRNAs (miRNAs). The ceRNA was constructed, then functional pathway enrichment analysis conducted. AT1DM-related established based on Human microRNA Disease Database carry out Meanwhile, T1DM-related pathways retrieved Comparative Toxicogenomics (CTD). Results In total, 847 41 lncRNAs, 38 miRNAs significantly expressed. consisted miRNAs, 24 mRNAs. Two ( hsa-miR-181a hsa-miR-1275 ) screened as build network, which genes considerably enriched seven pathways. Moreover, three overlapping pathways, including phosphatidylinositol signaling system (involving PIP4K2A , INPP4A PIP4K2C CALM1 ); dopaminergic synapse PPP2R5C insulin CBLB revealed by comparing with CTD, involved four lncRNAs LINC01278 TRG-AS1 MIAT GAS5-AS1 ). Conclusion identified may serve important regulators pathogenesis T1DM.