A booster dose of Delta × Omicron hybrid mRNA vaccine produced broadly neutralizing antibody against Omicron and other SARS-CoV-2 variants

• DOI: 10.1186/s12929-022-00830-1
• Publication Date: 2022-07-07T11:03:06Z
• Authors: I-Jung Lee; Cheng-Pu Sun; Ping-Yi Wu; Yu-Hua Lan; I-Hsuan Wang; Wen-Chun Liu; Joyce Pei-Yi Yuan; Yu-Wei Chang; Sheng-Che Tseng; Szu-I Tsung; Yu-Chi Chou; Monika Kumari; Yin-Shiou Lin; Hui-Feng Chen; Tsung-Yen Chen; Chih-Chao Lin; Chi-Wen Chiu; Chung-Hsuan Hsieh; Cheng-Ying Chuang; Chao-Min Cheng; Hsiu-Ting Lin; Wan-Yu Chen; Fu-Fei Hsu; Ming-Hsiang Hong; Chun-Che Liao; Chih-Shin Chang; Jian-Jong Liang; Hsiu-Hua Ma; Ming-Tsai Chiang; Hsin-Ni Liao; Hui-Ying Ko; Liang-Yu Chen; Yi-An Ko; Pei-Yu Yu; Tzu-Jing Yang; Po-Cheng Chiang; Shang-Te Hsu; Yi-Ling Lin; Chong-Chou Lee; Han-Chung Wu; Mi-Hua Tao
• Keywords: Variants of concern; 0301 basic medicine; Vaccines, Synthetic; SARS-CoV-2; Research; R; COVID-19; Omicron vaccine; Antibodies, Viral; Antibodies, Neutralizing; 3. Good health; Mice; 03 medical and health sciences; mRNA vaccine; Medicine; Animals; Humans; mRNA Vaccines; Hybrid vaccine; Broadly Neutralizing Antibodies

With the continuous emergence of new SARS-CoV-2 variants that feature increased transmission and immune escape, there is an urgent demand for a better vaccine design will provide broader neutralizing efficacy.We report mRNA-based using engineered "hybrid" receptor binding domain (RBD) contains all 16 point-mutations shown in currently prevailing Omicron Delta variants.A booster dose hybrid mice previously immunized with wild-type RBD induced high titers broadly antibodies against tested concern (VOCs). In naïve mice, generated strong Omicron-specific as well low but significant other VOCs. Hybrid also elicited CD8+/IFN-γ+ T cell responses conserved epitope present wild type VOCs.These results demonstrate inclusion different antigenic mutations from various feasible approach to develop cross-protective vaccines.