Angiogenic content of microparticles in patients with diabetes and coronary artery disease predicts networks of endothelial dysfunction

• DOI: 10.1186/s12933-022-01449-0
• Publication Date: 2022-02-02T15:02:54Z
• Authors: Isra Marei; Omar Chidiac; Binitha Thomas; Jennifer Pasquier; Soha Dargham; Amal Robay; Muneera Vakayil; Mohammad Jameesh; Christopher Triggle; Arash Rafii; Amin Jayyousi; Jassim Al Suwaidi; Charbel Abi Khalil
• Keywords: CD31; Endothelial Dysfunction; Angiology

Abstract Background Elevated endothelial microparticles (EMPs) levels are surrogate markers of vascular dysfunction. We analyzed EMPs with apoptotic characteristics and assessed the angiogenic contents in blood patients type 2 diabetes (T2D) according to presence coronary artery disease (CAD). Methods A total 80 participants were recruited equally classified as (1) healthy without T2D, (2) T2D cardiovascular complications, (3) chronic (CAD), (4) acute syndrome (ACS). MPs isolated from peripheral circulation, characterized using flow cytometry CD42 CD31. CD62E was used determine EMPs’ apoptotic/activation state. content extracted profiled an angiogenesis array. Results Levels CD42- CD31 + significantly increased ACS (257.5 ± 35.58) when compared subjects (105.7 12.96, p < 0.01). There no significant difference comparing CAD. The ratio CD42-CD62 +/CD42-CD31 reduced all patients, further reduction CAD, reflecting a release by cells. full population analyzed. It revealed differential expression 5 factors diabetes, including TGF-β1, PD-ECGF, platelet factor 4, serpin E1, thrombospondin 1. Ingenuity Pathway Analysis that those five differentially expressed molecules, mainly inhibit key pathways involved normal function. Further comparison three groups controls CAD identified networks cell Interestingly, DDP-IV only protein between diabetes. Conclusion Our data showed apoptosis-induced is irrespective having highest levels. interact indicate