Biosynthesis and engineering of kaempferol in Saccharomyces cerevisiae

• DOI: 10.1186/s12934-017-0774-x
• Publication Date: 2017-09-26T13:25:11Z
• Authors: Lijin Duan; Wentao Ding; Xiaonan Liu; Xiaozhi Cheng; Jing Cai; Erbing Hua; Huifeng Jiang
• Keywords: Metabolic Engineering

Kaempferol is a flavonol with broad bioactivity of anti-oxidant, anti-cancer, anti-diabetic, anti-microbial, cardio-protective and anti-asthma. Microbial synthesis kaempferol promising strategy because the low content in primary plant source. In this study, biosynthesis pathway was constructed budding yeast Saccharomyces cerevisiae to produce de novo, several biological measures were taken for high production. Firstly, efficient synthases (FLS) from Populus deltoides introduced into biosynthetic kaempferol. Secondly, S. recombinant novo kaempferol, which generated about 6.97 mg/L glucose. To further promote production, acetyl-CoA overexpressed p-coumarate supplied as substrate, improved titer by 23 120%, respectively. Finally, fed-batch process developed better fermentation performance, production reached 66.29 40 h. The our engineered 2.5 times highest reported titer. Our study provides possible using microbial cell factory.