Engineering xylose metabolism for production of polyhydroxybutyrate in the non-model bacterium Burkholderia sacchari

• DOI: 10.1186/s12934-018-0924-9
• Publication Date: 2018-05-15T09:03:30Z
• Authors: Linda P. Guamán; Carlos Barba-Ostria; Fuzhong Zhang; Edmar R. Oliveira...; José Gregório C. ...; Luiziana F. Silva
• Keywords: Polyhydroxybutyrate; Xylose metabolism; Bioproduction; Industrial microbiology

Despite its ability to grow and produce high-value molecules using renewable carbon sources, two main factors must be improved use Burkholderia sacchari as a chassis for bioproduction at an industrial scale: first, the lack of molecular tools engineer this organism second, inherently slow growth rate poly-3-hydroxybutyrate [P(3HB)] production xylose. In work, we have addressed both factors.First, adapted set BglBrick plasmids showed tunable expression in B. sacchari. Finally, assessed P(3HB) through overexpression xylose transporters, catabolic or regulatory genes. Overexpression xylR significantly (55.5% improvement), polymer yield (77.27% resulted 71% cell dry weight P(3HB).These values are unprecedented accumulation sole source highlight importance precise control improving utilization hemicellulosic sugars