Co-culture of Bacillus amyloliquefaciens ACCC11060 and Trichoderma asperellum GDFS1009 enhanced pathogen-inhibition and amino acid yield

• DOI: 10.1186/s12934-018-1004-x
• Publication Date: 2018-10-03T07:21:02Z
• Authors: Qiong Wu; Mi Ni; Kai Dou; Jun Tang; Jianhong Ren; Chuanjin Yu; Jie Chen
• Keywords: Bacillus amyloliquefaciens; Antibiosis; Trichoderma; Bacillus (shape)

Bacillus spp. are a genus of biocontrol bacteria widely used for antibiosis, while Trichoderma fungi that abundantly explored. In this study, liquid co-cultivation these two organisms was tried firstly.Through chromatography-mass spectrometry/mass spectrometry (LC-MS/MS), it discovered with an inoculation in the ratio 1.9:1, antimicrobial effect co-cultured fermentation liquor amyloliquefaciens ACCC11060 and asperellum GDFS1009 found to be significantly higher than pure-cultivation. A raise synthesis substances contributed significant increase. Additionally, co-culture 1:1 enhance production specific amino acids. This technique could further explored either large scale acids or serve as theoretical base generation certain rare acids.This work clearly demonstrated B. T. produce more