CircDOCK1 promotes the tumorigenesis and cisplatin resistance of osteogenic sarcoma via the miR-339-3p/IGF1R axis

• DOI: 10.1186/s12943-021-01453-0
• Publication Date: 2021-12-07T08:02:52Z
• Authors: Shenglong Li; Fei Liu; Ke Zheng; Wei Wang; Enduo Qiu; Yi Pei; Shuang Wang; Jiaming Zhang; Xiaojing Zhang
• Keywords: Circular RNA

Abstract Background Circular RNAs (circRNAs), a class of noncoding (ncRNAs), may modulate gene expression by binding to miRNAs. Additionally, recent studies show that circRNAs participate in some pathological processes. However, there is large gap the knowledge about circDOCK1 and its biological functions osteogenic sarcoma (OS). Methods Differentially expressed OS cell lines tissues were identified circRNA microarray analysis quantitative real-time PCR (qRT–PCR). To explore actions vivo vitro, was knocked down or overexpressed. assess regulatory associations among miR-339-3p, IGF1R, we performed rescue experiments, RNA immunoprecipitation (RIP), pulldown assays dual-luciferase assays. Moreover, apoptosis reveal effects circDOCK1/miR-339-3p/IGF1R axis on cisplatin sensitivity. Results CircDOCK1 remained stable cytoplasm higher cells than corresponding controls. Overexpression increased oncogenicity malignant transformation vitro. In U2OS MG63 lines, modulated tumor progression regulating IGF1R through sponging miR-339-3p. U2OS/DDP MG63/DDP sensitivity regulated via miR-339-3p/IGF1R axis. Conclusions can promote regulate Thus, be key mechanism therapeutic target OS.