Exploring cancer metastasis prevention strategy: interrupting adhesion of cancer cells to vascular endothelia of potential metastatic tissues by antibody-coated nanomaterial

• DOI: 10.1186/s12951-015-0072-x
• Publication Date: 2015-02-02T13:39:03Z
• Authors: Jingjing Xie; Haiyan Dong; Hongning Chen; Rongli Zhao; Patrick J Sinko; Weiyu Shen; Jichuang Wang; Yusheng Lu; Xiang Yang; Fangwei Xie; Lee Jia
• Keywords: Conjugate

Cancer metastasis caused by circulating tumor cells (CTCs) accounts for 90% cancer-related death worldwide. Blocking the circulation of CTCs in bloodstream and their hetero-adhesion to vascular endothelia distant metastatic organs may prevent cancer metastasis. Nanomaterial-based intervention with adhesion between has not been reported. Driven novel idea that multivalent conjugation EpCAM Slex antibodies dendrimer surface enhance capacity specificity nanomaterial conjugates capturing down-regulating colorectal CTCs, we conjugated antibodies, examined dual antibody-coated roles interrupting CTCs-related The was synthesized characterized. specifically bound captured colon SW620. conjugate inhibited cells' viability fibronectin (Fn)-coated substrate or human umbilical vein endothelial (HUVECs) a concentration-dependent manner. In comparison SW480 LoVo cell lines, activity SW620 Fn-coated HUVECs were more antibody because higher levels on surface. substrate, about 60-70%. showed inhibition significant than its corresponding single conjugates. present study provides new evidence coating nanomaterials one against effectively interfere interaction HUVECs.