Chitosan-DNA nanoparticles enhanced the immunogenicity of multivalent DNA vaccination on mice against Trueperella pyogenes infection

• DOI: 10.1186/s12951-018-0337-2
• Publication Date: 2018-01-29T06:38:49Z
• Authors: Ting Huang; Xuhao Song; Jie Jing; Kelei Zhao; Yongmei Shen; Xiuyue Zhang; Bisong Yue
• Keywords: CpG Oligodeoxynucleotide

Trueperella pyogenes is a commensal and opportunistic pathogen that normally causes mastitis, liver abscesses pneumonia of economically important livestock. To develop efficacious potent vaccine against T. pyogenes, chimeric gene DNA vaccines were constructed encapsulated in chitosan nanoparticles (pPCFN-CpG-CS-NPs).The pPCFN-CpG-CS-NPs consists the plo, cbpA, fimA, nanH CpG ODN1826. It was produced with good morphology, high stability, mean diameter 93.58 nm, zeta potential + 5.27 mV. Additionally, encapsulation confirmed to protect plasmid from DNase I digestion. The immunofluorescence assay indicated four-chimeric could synchronously express HEK293T cells maintain bioactivity. Compared mice immunized control plasmid, vivo immunization showed had better immune responses, release prolonged. Importantly, significantly highly virulent TP7 infection.This study indicates chitosan-DNA are candidates infection provides strategies for further development novel nanoparticles.