Targeted theranostic photoactivation on atherosclerosis

• DOI: 10.1186/s12951-021-01084-z
• Publication Date: 2021-10-24T20:02:40Z
• Authors: Joon Woo Song; Jae Won Ahn; Min Woo Lee; Hyun Jung Kim; Dong Oh Kang; Ryeong Hyun Kim; Un Gyo Kang; Yeon Hoon Kim; Jeongmoo Han; Ye Hee Park; Hyeong Soo Nam; Hongki Yoo; Kyeongsoon Park; Jin Won Kim
• Keywords: efferocytosis; Atheroma; Scavenger Receptor

Photoactivation targeting macrophages has emerged as a therapeutic strategy for atherosclerosis, but limited targetable ability of photosensitizers to the lesions hinders its applications. Moreover, molecular mechanistic insight phototherapeutic effects on atheroma is still lacking. Herein, we developed macrophage near-infrared fluorescence (NIRF) emitting phototheranostic agent by conjugating dextran sulfate (DS) chlorin e6 (Ce6) and estimated feasibility in murine atheroma. Also, mechanisms DS-Ce6 atherosclerosis were investigated.The efficiently internalized into activated foam cells via scavenger receptor-A (SR-A) mediated endocytosis. Customized serial optical imaging-guided photoactivation light illumination reduced both burden inflammation models. Immuno-fluorescence -histochemical analyses revealed that produced prominent increase macrophage-associated apoptotic bodies 1 week after laser irradiation induced autophagy with Mer tyrosine-protein kinase expression early day 1, indicative an enhanced efferocytosis atheroma.Imaging-guided was able vivo detect inflammatory activity well simultaneously reduce plaque harmonic contribution apoptosis, autophagy, lesional efferocytosis. These results suggest nanoagents will be promising theranostic high-risk