Construction of lymph nodes-targeting tumor vaccines by using the principle of DNA base complementary pairing to enhance anti-tumor cellular immune response

• DOI: 10.1186/s12951-024-02498-1
• Publication Date: 2024-05-08T09:02:40Z
• Authors: Yongchao Zha; Li Fu; Zonghua Liu; Jiansheng Lin; Linghong Huang
• Keywords: Tumor Antigen

Abstract Tumor vaccines, a crucial immunotherapy, have gained growing interest because of their unique capability to initiate precise anti-tumor immune responses and establish enduring memory. Injected tumor vaccines passively diffuse the adjacent draining lymph nodes, where residing antigen-presenting cells capture present antigens T cells. This process represents initial phase response constitutes pivotal determinant effectiveness. Nevertheless, granularity paradox, arising from different requirements between passive targeting delivery nodes uptake by cells, diminishes efficacy node-targeting vaccines. study addressed this challenge employing vaccine formulation with tunable, controlled particle size. Manganese dioxide (MnO 2 ) nanoparticles were synthesized, loaded ovalbumin (OVA), modified A 50 or 20 DNA single strands obtain MnO /OVA/A /OVA/T , respectively. Administering sequentially, upon reaching two converge simultaneously aggregate into -T particles through base pairing. enhances both antigen delivery. In vitro in vivo studies demonstrated that, combined vaccine, comprising exhibited robust immunization effects remarkable melanoma animal models. The strategy controlling size consequently improving presentation efficiency via base-pairing principle, provides novel concepts for development efficient Graphical