Antimicrobial activity of HL-60 cells compared to primary blood-derived neutrophils against Staphylococcus aureus

• DOI: 10.1186/s12952-017-0067-2
• Publication Date: 2017-02-02T12:31:11Z
• Authors: Ragheda Yaseen; Stefanie Blodkamp; Petra Lüthje; Friederike Reuner; Lena Völlger; Hassan Y. Naim; Maren von Köckrit...
• Keywords: Neutrophil Extracellular Traps; Blood cell

The human leukemia cell line HL-60 is considered an alternative culture model to study neutrophil differentiation and migration. aim of this was characterize the suitability cells differentiated neutrophil-like (nHL-60) as substitute for blood-derived neutrophils investigate interaction with Staphylococcus aureus.For purpose, antimicrobial activity, bacterial uptake, production reactive oxygen species release extracellular traps (NETs) by nHL-60 were analyzed compared primary using aureus important animal pathogen.Overall, activities distinctly lower neutrophils. Furthermore, well NET formation clearly impaired in cells.This indicates that are limited usage functions against aureus.