Cisplatin-induced mesenchymal stromal cells-mediated mechanism contributing to decreased antitumor effect in breast cancer cells

• DOI: 10.1186/s12964-016-0127-0
• Publication Date: 2016-01-12T03:53:37Z
• Authors: Svetlana Skolekova; Miroslava Matuskova; Martin Bohac; Lenka Toro; Erika Durinikova; Silvia Tyciakova; Lucia Demkova; Jan Gursky; Lucia Kucerova
• Keywords: 0301 basic medicine; Interleukin-6; Research; Interleukin-8; Antineoplastic Agents; Apoptosis; Breast Neoplasms; Mesenchymal Stem Cells; Cell Biology; 3. Good health; 03 medical and health sciences; Drug Resistance, Neoplasm; Cell Line, Tumor; Tumor Microenvironment; Humans; Female; Breast; Cisplatin; Molecular Biology; Cellular Senescence

Cells of the tumor microenvironment are recognized as important determinants biology. The adjacent non-malignant cells can regulate drug responses cancer by secreted paracrine factors and direct interactions with cells. Human mesenchymal stromal (MSC) actively contribute to microenvironment. Here we focused on their response chemotherapy during treatment these become affected. We have shown that secretory phenotype behavior influenced cisplatin differs from naïve MSC. MSC were more resistant concentrations cisplatin, which was cytotoxic for They did not undergo apoptosis, but a part population underwent senescence. However, pretreatment led changes in phosphorylation profiles many kinases also increased secretion IL-6 IL-8 cytokines. These cytokine profile chemoresistance stemness breast Taken together here suggest exposure chemoresistant leads substantial alterations might lead promotion acquired microenvironment-mediated stemness.