Interleukin-1 receptor antagonist inhibits metastatic potential by down-regulating CXCL12/CXCR4 signaling axis in colorectal cancer

• DOI: 10.1186/s12964-021-00804-0
• Publication Date: 2021-12-20T11:12:19Z
• Authors: Jiachi Ma; Wanqing Liang; Yaosheng Qiang; Lei Li; Jun Du; Chengwu Pan; Bangling Chen; Chensong Zhang; Yuzhong Chen; Qingkang Wang
• Keywords: Interleukin 8; CXCR4 antagonist

Abstract Background The aim of this study was to investigate the co-operative role CXCR4/CXCL12 axis and IL-1Ra in metastatic processes mechanism by interactions between colorectal cancer cells stromal their microenvironment. Methods Expression IL-1α, interleukin-1 receptor type I (IL-1 RI), CXCL12 CXCR4 mRNA proteins were determined RT-PCR Western blot. effect secreted level on fibroblasts measured ELISA. regulate potential evaluated proliferation, invasion angiogenesis assays, respectively, which assays used an vitro system consisting co-cultured cells. Results IL-1α expressed high liver cell lines (HT-29 WiDr). cell-derived rIL-1α significantly promoted expression fibroblasts, enhancing can be inhibited ( P < 0.01) . not only enhanced migration proliferation human umbilical vein endothelial cells, but also 0.01). Furthermore, liver-metastatic line (HT-29), secretes compared low (CaCo-2), does produce On contrary, inhibit migration, Conclusion Autocrine paracrine co-enhances cells; via decrease IL-1α/CXCR4/CXCL12 signaling pathways. Graphical