Fusobacterium nucleatum triggers proinflammatory cell death via Z-DNA binding protein 1 in apical periodontitis

• DOI: 10.1186/s12964-022-01005-z
• Publication Date: 2022-12-20T11:04:43Z
• Authors: Hui Liu; Yuxuan Liu; Wei Fan; Bing Fan
• Keywords: Pyroptosis; Fusobacterium nucleatum; Proinflammatory cytokine

Abstract Background Z-DNA binding protein 1 (ZBP1) is a vital innate immune sensor that regulates inflammation during pathogen invasion. ZBP1 may contribute to pyroptosis, apoptosis and necroptosis in infectious diseases. In this study, Fusobacterium nucleatum ( F. ) infection caused periapical through proinflammatory cell death was involved regulating the inflammatory activities by apical periodontitis (AP). Methods Human tissues were tested fluorescent situ hybridization, immunohistochemical staining, immunofluorescence quantitative real-time PCR (qRT‒PCR) western blotting. -infected extracellular vesicles -EVs)-treated RAW264.7 cells used detect expression of cytokines different mechanisms qRT‒PCR detected qRT‒PCR, blotting, staining. Furthermore, inhibited siRNA pathways, including apoptosis, necroptosis, measured cells. Results AP tissues. polarized M1 phenotype, accompanied cytokine production. High levels GSDME (gasdermin E)-mediated caspase-3-mediated MLKL-mediated (PANoptosis) identified inhibition reduced secretion occurrence PANoptosis. Conclusion The present study previously unknown role -induced activation.