Integrin-linked kinase mRNA expression in circulating mononuclear cells as a biomarker of kidney and vascular damage in experimental chronic kidney disease

• DOI: 10.1186/s12964-024-01646-2
• Publication Date: 2024-05-11T14:01:39Z
• Authors: Sofía Campillo; Elena Gutiérrez-C...; Susana García-Mir...; Mercedes Griera; Loreto Fernández ...; Sergio de Frutos; Diego Rodríguez-P...; Laura Calleros
• Keywords: Integrin-linked kinase; Blood urea nitrogen

Abstract Background Traditional biomarkers of chronic kidney disease (CKD) detect the in its late stages and hardly predict associated vascular damage. Integrin-linked kinase (ILK) is a scaffolding protein serine/threonine that plays multiple roles several pathophysiological processes during renal However, involvement ILK as biomarker CKD problems remains to be fully elucidated. Methods was induced by an adenine-rich diet for 6 weeks mice. We used inducible knockdown mice (cKD-ILK) model decrease expression. content mice's peripheral blood mononuclear cells (PBMCs) determined correlated with function parameters expression fibrosis inflammation markers aortic tissues. Also, five miRNAs target analyzed whole Results The adenine increased PBMCs, cortex, aortas, creatinine urea nitrogen concentrations plasma WT mice, while these increases were not observed cKD-ILK Furthermore, PBMCs directly markers. Finally, adenine-fed although only four nitrogen, those, three downregulated Conclusions ILK, circulating cells, could potential CKD-associated Graphical reflects damage experimental model.