Abroma augusta L. (Malvaceae) leaf extract attenuates diabetes induced nephropathy and cardiomyopathy via inhibition of oxidative stress and inflammatory response

• DOI: 10.1186/s12967-014-0364-1
• Publication Date: 2015-01-15T12:51:36Z
• Authors: Ritu Khanra; Saikat Dewanjee; Tarun K Dua; Ranabir Sahu; Moumita Gangopadhyay; Vincenzo De Feo; Muhammad Zia-Ul-Haq
• Keywords: Diabetic Cardiomyopathy

Abroma augusta L. (Malvaceae) leaf is traditionally used to treat diabetes in India and Southern Asia. Therefore, current study was performed evaluate the protective effect of defatted methanol extract A. leaves (AA) against type 2 mellitus (T2DM) its associated nephropathy cardiomyopathy experimental rats.Antidiabetic activity AA extracts (100 200 mg/kg, p.o.) measured streptozotocin-nicotinamide induced diabetic (T2D) rat. Fasting blood glucose level (at specific interval) serum biochemical markers (after sacrifice) were measured. Redox status, transcription levels signal proteins (NF-κB PKCs), mitochondria dependent apoptotic pathway (Bad, Bcl-2, caspase cascade) histological studies kidneys hearts controls treated rats.Phytochemical screening revealed presence taraxerol, flavonoids phenolic compounds AA. T2D rats showed significantly (p < 0.01) elevated fasting level. Alteration lipid profile release membrane bound enzymes like lactate dehydrogenase creatine kinase, which ensured participation hyperlipidemia cell disintegration pathophysiology. T2DM caused alteration related complications. altered redox decreased intracellular NAD ATP concentrations renal myocardial tissues rats. Investigating molecular mechanism, activation PKC isoforms observed selected tissues. also exhibited an up-regulation NF-κB increase pro-inflammatory cytokines (IL-1β, IL-6 TNF-α) cardiac The However, Oral administration at doses 100 mg/kg body weight per day could reduce hyperglycemia, hyperlipidemia, disintegration, oxidative stress, vascular inflammation prevented stress signaling cascades leading death. Histological supported characteristics AA.Results suggest that offer prophylactic role reno- cardio- toxicity.