Exosomes released from human induced pluripotent stem cells-derived MSCs facilitate cutaneous wound healing by promoting collagen synthesis and angiogenesis

• DOI: 10.1186/s12967-015-0417-0
• Publication Date: 2015-01-31T12:56:45Z
• Authors: Jieyuan Zhang; Junjie Guan; Xin Niu; Guowen Hu; Shangchun Guo; Qing Li; Zongping Xie; Changqing Zhang; Yang Wang
• Keywords: Dermal fibroblast

Human induced pluripotent stem cell-derived mesenchymal cells (hiPSC-MSCs) have emerged as a promising alternative for cell transplantation therapy. Exosomes derived from (MSC-Exos) can play important roles in repairing injured tissues. However, to date, no reports demonstrated the use of hiPSC-MSC-Exos cutaneous wound healing, and little is known regarding their underlying mechanisms tissue repair.hiPSC-MSC-Exos were injected subcutaneously around sites rat model efficacy was assessed by measuring closure areas, histological immunofluorescence examinations. We also evaluated vitro effects on both proliferation migration human dermal fibroblasts umbilical vein endothelial (HUVECs) cell-counting scratch assays, respectively. The exosomes fibroblast collagen elastin secretion studied enzyme-linked immunosorbent assays quantitative reverse-transcriptase-polymerase chain reaction (qRT-PCR). In capillary network formation determined tube-formation assays.Transplanting resulted accelerated re-epithelialization, reduced scar widths, promotion maturity. Moreover, not only promoted generation newly formed vessels, but maturation sites. found that stimulated HUVECs dose-dependent manner vitro. Similarly, Type I, III mRNA expression tube increased with increasing concentrations.Our findings suggest facilitate healing promoting synthesis angiogenesis. These data provide first evidence potential treating wounds.