Let-7a inhibits migration, invasion and epithelial-mesenchymal transition by targeting HMGA2 in nasopharyngeal carcinoma

• DOI: 10.1186/s12967-015-0462-8
• Publication Date: 2015-03-30T06:24:33Z
• Authors: Aibing Wu; Kunpeng Wu; Jinmei Li; Yanli Mo; Yanming Lin; Yuzhou Wang; Xiang Shen; Shujun Li; Lixia Li; Zhixiong Yang
• Keywords: HMGA2

Let-7a has been shown to play important roles in nasopharyngeal carcinoma (NPC) cell proliferation and apoptosis, but little is known about the function mechanism of let-7a metastasis. We aimed investigate metastasis clarified regulation high mobility group A2 (HMGA2) by let-7a. The expression levels HMGA2 were examined NPC clinical specimens using quantitative reverse transcription-PCR (RT-qPCR). was confirmed as a target through luciferase reporter assays, RT-qPCR, Western blotting. Furthermore, regulating cells biological properties including proliferation, migration, invasion epithelial-mesenchymal transition (EMT) process analyzed with mimics si-HMGA2 transfected cells. Our study demonstrated that downregulated inversely associated stage, T classification N classification, upregulated directly stage patients NPC. Moreover, there an inverse correlation between patient. In addition, negatively regulated at posttranscriptional level via binding site HMGA2-3′UTR. synthetic suppressed EMT knockdown consistent effects downregulates protein expression, which suppress process. could serve potential diagnostic marker therapeutic for