The effects of two common edible herbs, Ipomoea aquatica and Enhydra fluctuans, on cadmium-induced pathophysiology: a focus on oxidative defence and anti-apoptotic mechanism

• DOI: 10.1186/s12967-015-0598-6
• Publication Date: 2015-07-27T10:19:57Z
• Authors: Tarun K Dua; Saikat Dewanjee; Ritu Khanra; Niloy Bhattacharya; Bhuvan Bhaskar; Muhammad Zia-Ul-Haq; Vincenzo De Feo
• Keywords: Phytochemical; Viability assay; Hepatoprotection

Ipomoea aquatica (Convolvulaceae) and Enhydra fluctuans (Asteraceae), two aquatic vegetables, are traditionally used against heavy metal toxicity in traditional medicines India. The present study aimed to explore the protective role of edible (aqueous) extracts I. (AEIA) E. (AEEF) Cd-intoxication. were chemically standardized by spectroscopic HPLC analysis. cytoprotective roles AEIA AEEF measured on mouse hepatocytes. effect redox status after incubating hepatocytes with CdCl2 (30 μM) along or (400 μg/ml). effects expressions apoptotic signal proteins estimated. vivo assay mice. Haematological, serum biochemical, tissue status, Cd bioaccumulation histological parameters evaluated estimate (100 mg/kg) (4 intoxication. Phytochemical analysis revealed presence substantial quantities phenolics, flavonoids, saponins, carbohydrates ascorbic acid AEEF. treated murine showed a gradual reduction cell viability concentration dependent manner an IC50 ~30 μM. exhibited significantly enhanced levels (p < 0.01) ROS production, lipid peroxidation, protein carbonylation NADPH oxidase concomitant depletion antioxidant enzymes GSH. However, treatment restored aforementioned near normalcy. Besides, counteracted 0.05–0.01) mediated alteration transcription viz. Bcl-2, BAD, Cyt-C, Caspases, Fas Bid. In bioassay, caused high oxidative stress liver, kidney, heart, brain testes addition, haematological biochemical altered animals. Simultaneous administration could restore tested near-normal status. would offer overall via counteracting and/or promoting elimination chelating.