RETRACTED ARTICLE: TIPE2 suppresses progression and tumorigenesis of esophageal carcinoma via inhibition of the Wnt/β-catenin pathway

• DOI: 10.1186/s12967-018-1383-0
• Publication Date: 2018-01-17T12:25:08Z
• Authors: Linan Zhu; Xudong Zhang; Xiaorui Fu; Zhaoming Li; Zhenchang Sun; Jingjing Wu; Xinhua Wang; Feng Wang; Xiangke Li; Songtao Niu; Mengjie Ding; Zhenzhen Yang; Wanqiu Yang; Meifeng Yin; Lei Zhang; Mingzhi Zhang
• Keywords: Male; Esophageal Neoplasms; Carcinogenesis; Proliferation; Down-Regulation; Mice, Nude; Apoptosis; 03 medical and health sciences; Cell Line, Tumor; Wnt/β-catenin pathway; Animals; Humans; Neoplasm Invasiveness; Wnt Signaling Pathway; Aged; Cell Proliferation; Mice, Inbred BALB C; 0303 health sciences; Research; R; Intracellular Signaling Peptides and Proteins; Middle Aged; Xenograft Model Antitumor Assays; TIPE2; 3. Good health; Gene Expression Regulation, Neoplastic; Esophageal carcinoma; Tumorigenesis; Disease Progression; Medicine; Female

Abstract Background Esophageal carcinoma is the eighth prevalent malignancy and ranks sixth in carcinoma-related death worldwide. Tumor necrosis factor-α-induced protein-8 like-2 (TIPE2) has been identified as a tumor suppressor multiple carcinomas. However, its roles molecular mechanisms underlying esophageal progression are still undefined till now. Methods RT-qPCR assay was employed to detect expression of TIPE2 mRNA. protein measured by using western blot assay. Ad-V Ad-TIPE2 adenoviruses were constructed overexpress TIPE2. The effects overexpression on cell proliferation, invasion apoptosis assessed MTT Edu incorporation assays, transwell flow cytometry analysis, respectively. effect xenograft growth determined measuring volume weight, together with immunohistochemistry Wnt/β-catenin signaling pathway evaluated detecting levels β-catenin, c-Myc cyclinD1 EC9076 cells tumors carcinoma. Results downregulated tissues cells. Adenovirus-mediated suppressed proliferation invasion, induced Enforced inhibited vivo, evidenced reduced volume, weight proliferating nuclear antigen expression. Overexpression vitro vivo. Conclusions These results suggest that tumorigenesis via inhibition pathway.