Distinct alterations of CD68+CD163+ M2-like macrophages and myeloid-derived suppressor cells in newly diagnosed primary immune thrombocytopenia with or without CR after high-dose dexamethasone treatment

• DOI: 10.1186/s12967-018-1424-8
• Publication Date: 2018-03-02T04:22:19Z
• Authors: Xia Shao; Boting Wu; Luya Cheng; Feng Li; Yanxia Zhan; Chanjuan Liu; Lili Ji; Zhihui Min; Yang Ke; Lihua Sun; Hao Chen; Yunfeng Cheng
• Keywords: CD163; Myeloid-derived Suppressor Cell; M2 Macrophage; CD68

Although impaired myeloid-derived suppressor cells (MDSCs) recently have been studied in immune thrombocytopenia (ITP), another cell population signified as M2 macrophages has not investigated properly ITP patients. In the present study, we intended to determine features of circulating M2-like macrophages, examine its relationship with MDSCs, and explore their prognostic values ITP. Peripheral blood mononuclear from healthy controls primary patients were isolated test MDSCs. The macrophage defined CD68+CD163+ MDSC CD11b+CD33+HLA-DR− determined by flow cytometry. Plasma inflammatory cytokines measured multiplex ELISA. percentages MDSCs found be expanded newly diagnosed ITP, especially among those complete response (CR) group (p < 0.0001). Positive linear correlation was verified between same also CR group. After treatment, both increased significantly group, while PR + NR manifested a significant numeric decrease but only moderate macrophages. MIP-1α/CCL3 negatively correlated MCP-1 possessed positive eotaxin-1/CCL11 interleukin-1β (IL-1β) findings indicated critical roles them might related factors-mediated bidirectional interactions or partially due similar background patterns during differentiation. MIP-1α/CCL3, MCP-1, IL-1β play role expansion patients, which deserves further investigation.